Apparatus and method for volumetric capillary cytometry
US5547849A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 2, 1994 |
| Grant date | Aug 20, 1996 |
| Priority date | — |
| Expiry date | May 2, 2014 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N15/1468
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The apparatus and method of the present invention disclose a scanning imaging cytometer wherein an unprocessed biological fluid sample is reacted with a fluorescently-labeled binding agent. The reacted sample undergoes minimal processing before it is placed into a capillary tube. The sample is optically scanned and fluorescence excitation is recorded from a plurality of columnar regions of the capillary tube, each columnar region generally defined by the spot size of the excitation beam and the depth dimension of the capillary tube. A spatial filter of a sufficient pinhole diameter is selected to allow simultaneous volumetric detection of all fluorescent targets in each columnar region.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.