Process for determining hirudin and synthetic thrombin inhibitors

US5547850A · kind A · utility

Assignee

• Max-Planck-Gesellschaft · DE

Inventors

• Gotz Nowak · Erfurt, DE
• Elke Bucha · Erfurt, DE
• Jutta Hoffman · Erfurt, DE

Key dates

• Filing date: Oct 5, 1994
• Grant date: Aug 20, 1996
• Expiry date: Oct 5, 2014

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/974
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides a process for determining the content of hirudin or of a synthetic thrombin inhibitor in the blood. A prothrombin intermediate, or a compound that splits prothrombin into meizothrombin, or a mixture thereof, is added to a blood sample and a measurement is taken of the time that elapses between the addition of the reagent to the sample and the beginning of coagulation. Comparison is then made, where necessary, with a standard calibration curve.