Sulphocoumarin-containing nucleotides and their use in processes for detecting nucleic acids

US5547860A · kind A · utility

Assignee

• BAYER HEALTHCARE LLC · DE

Inventors

• Jürgen Köcher · Cologne, DE
• Wolfgang Springer · Wuppertal, DE
• Eberhard Kuckert · West Haven, US
• Thomas Böcker · Leichlingen, DE

Key dates

• Filing date: Jan 21, 1994
• Grant date: Aug 20, 1996
• Expiry date: Jan 21, 2014

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/533
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention relates to the preparation, as substituents, of nucleotides possessing a fluorescent coumarin residue, the enzymic incorporation of these nucleotides into nucleic acids and the detection of nucleic acids of defined sequence by hybridization with a complementary, coumarin-labelled nucleic acid.