Somatotropins with alterations in the alpha-helix 1 region, and combinations with other mutations
US5548068A · kind A · utility
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12Claims
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Key dates
| Filing date | Dec 23, 1994 |
| Grant date | Aug 20, 1996 |
| Priority date | — |
| Expiry date | Dec 23, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/61
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to somatotropin analogues with amino acid changes in the alpha-helix 1 regions of said somatotropins, alone or in combination with mutations in the alpha-helix 3 and/or alpha-helix 2 regions, plus combinations with other changes to the native amino acid sequence of somatotropins. The resulting analogues are stable for formulation in sustained release formulations, while maintaining biological activity. Further, methods for conducting site-directed mutagenesis on DNA encoding proteins and/or polypeptide also are provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.