Prokaryotic expression in eukaryotic cells

US5550035A · kind A · utility

Assignee

• The Research Foundation of the State University of New York · US

Inventors

• Bernard Moss · Bethesda, US
• F. William Studier · Stony Brook, US
• Thomas R. Fuerst · Gaithersburg, US
• Edward Niles · Amherst, US

Key dates

• Filing date: Jan 26, 1994
• Grant date: Aug 27, 1996
• Expiry date: Jan 26, 2014

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/85
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A transient expression system is disclosed that utilizes bacteriophage RNA polymerase in the presence of a DNA-based cytoplasmic virus to facilitate expression of a foreign gene in the cytoplasm of a eukaryotic cell. A method of expressing a foreign gene in the cytoplasm of a eukaryotic cell is also disclosed which comprises incorporating into the cytoplasm a DNA-based cytoplasmic virus, a suitable carrier comprising a gene for an RNA polymerase which gene is foreign to the carrier and to the cells, and a suitable carrier comprising a functional, cistron including a foreign gene flanked by a promotor sequence which is recognized by the RNA polymerase.