Patent · US Expired

Method for separation and recovery of biological materials

US5552325A · kind A · utility

76Cited by
25References
8Claims
0Family size

Assignee

Inventors

Key dates

Filing dateJul 17, 1992
Grant dateSep 3, 1996
Priority date
Expiry dateJul 17, 2012

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10T436/255
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

Disclosed is a centrifuge tube containing a porous selection means for selectively separating and recovering biological substances from gels, broths, or solutions containing the same, and thus concentrating them, the tube comprising separate upper and lower containers and means for joining the two containers to form a unitary device, the bottom of the upper container comprising a porous selection means, preferably a membrane containing a particulate substance which will selectively bind the biological substances while allowing unbound substances to pass through from the upper to lower container while being centrifuged. A method for recovering the biological substances in high concentration using the centrifuge tube is also provided, comprising introducing said gels, broths, or solutions containing the desired biological substances, centrifuging the same until the desired substance is bound to the selection means, and eluting the bound material from the selection means to recover the substance in concentrated form.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.