Nucleic acid sequence amplification method, composition and kit
US5554516A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 2, 1993 |
| Grant date | Sep 10, 1996 |
| Priority date | — |
| Expiry date | Dec 2, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/689
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method, composition and kit for amplifying a target nucleic acid sequence under conditions of substantially constant temperature, ionic strength, and pH and using only a single promoter-primer. To effect the amplification, a supply of a single promoter-primer having a promoter and a primer complementary to the 3'-end of the target sequence, and a reverse transcriptase and an RNA polymerase are provided to a mixture including the target sequence; the amplification proceeds accordingly. The invention is useful for generating copies of a nucleic acid target sequence for purposes that include assays to quantitate specific nucleic acid sequences in clinical, environmental, forensic and similar samples, cloning and generating probes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.