Aseptic in vitro endomycorrhizal spore mass production
US5554530A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 4, 1994 |
| Grant date | Sep 10, 1996 |
| Priority date | — |
| Expiry date | Aug 4, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/911
- WIPO fieldFood chemistry
- WIPO sectorChemistry
Abstract
The present invention relates to a method of producing mycorrhizal fungal propagules in vitro in a two-compartment container having a gellified medium, which comprises the steps of: a) cultivating aseptically transformed dicotyledon root organs, capable of autonomous growth in vitro, in a first compartment containing a mineral minimal medium with sugar, wherein the medium is suitable for root growth; b) inoculating the transformed root organs with endomycorrhizal spores; and c) cultivating the inoculated transformed root organs for a time sufficient for the mycorrhizal fungi to transfer to a second root-free and root exudate-free compartment containing the mineral minimal medium of step a) and for the mass production of fungal propagules to occur in the second compartment.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.