Haloperoxidase acid optimum chemiluminescence assay system
US5556758A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | May 19, 1994 |
| Grant date | Sep 17, 1996 |
| Priority date | — |
| Expiry date | May 19, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/28
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A highly sensitive chemiluminescent indicator system is disclosed for determining the presence or amount of an analyte in a liquid sample. The new acid optimum chemiluminescent indicator system comprises haloperoxidase (halide:hydrogen peroxide oxidoreductase), halide, oxidant and chemiluminigenic substrate. The indicator system acts as a synthesizer of highly reactive singlet molecular oxygen (.sup.1 O.sub.2), which reacts with the chemiluminigenic substrate to yield an excited state, oxidized reaction product. The excited state reaction product then relaxes to a lower energy (e.g., ground) state with the emission of measurable light in an amount related to the amount of each of the reaction participants present in a reaction solution. Known, non-rate limiting amounts of three of the reaction participants are provided in an assay solution to determine the presence or amount of the fourth participant in a test sample. The fourth participant in the test sample may be the analyte of interest, or may be produced or consumed in the test sample through one or more preliminary reactions involving the ultimate analyte of interest, with the amount of the fourth participant being related to…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.