Coupled replication-translation methods and kits for protein synthesis
US5556769A · kind A · utility
19Cited by
3References
44Claims
0Family size
Inventors
Key dates
| Filing date | Aug 23, 1994 |
| Grant date | Sep 17, 1996 |
| Priority date | — |
| Expiry date | Aug 23, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P21/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Batch and continuous-flow cell-free methods for synthesizing protein wherein translation is coupled with replication of recombinant mRNA by an RNA-directed RNA polyerase, such as Q.beta. replicase. Transcription of a DNA template to produce the recombinant mRNA can be coupled additionally with coupled transcription-replication-translation methods, both batch and continuous. The invention also includes kits of reagents for synthesizing user-selected proteins according to methods of this invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.