Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification
US5556771A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 10, 1995 |
| Grant date | Sep 17, 1996 |
| Priority date | — |
| Expiry date | Feb 10, 2015 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Stabilized reverse transcriptase and RNA polymerase compositions for use in nucleic acid amplification. Compositions are provided for the stabilization of one or more enzymes in a single stabilized formulation. Additional compositions incorporate a dried, stabilized enzyme mixture together with necessary cofactors and enzyme substrates in a single container for use upon rehydration. Also disclosed are methods for making and using stabilized enzyme compositions and kits for nucleic acid amplification incorporating the disclosed compositions of reverse transcriptase and RNA polymerase lyophilized with a cryoprotectant stabilizing agent including trehalose and polyvinylpyrrolidone.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.