Process for detection of new polymorphic loci in a DNA sequence, nucleotide sequences forming hybridization probes and their applications
US5556955A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Sep 8, 1994 |
| Grant date | Sep 17, 1996 |
| Priority date | — |
| Expiry date | Sep 8, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/172
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for detection of a new polymorphic locus in a DNA sequence is characterized by the following stages. Polynucleotides are prepared by forming an unspecified oligonucleotide including 5 to 10 bases which are repeated 5 to 100 times. A genomic bank is explored by hybridization under low stringency conditions using the prepared polynucleotides which are labelled. The clones that react with the polynucleotides are analyzed by the Southern method. The restriction fragments detected by the polynucleotides are purified and labelled. The genomic DNA of non-consanguineous individuals is analyzed by the Southern method under highly stringent hybridization conditions, with the obtained restriction fragments. The restriction fragments corresponding to new polymorphic loci are selected.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.