Method of amplification using intermediate renaturation step
US5559013A · kind A · utility
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2References
15Claims
0Family size
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Key dates
| Filing date | Jun 22, 1994 |
| Grant date | Sep 24, 1996 |
| Priority date | — |
| Expiry date | Jun 22, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/686
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for amplification and detection of a low copy target nucleic acid includes coamplification of a high copy target nucleic acid. After a number of conventional amplification cycles which include a denaturation step, several cycles are carried out during which the denatured products are renatured for a brief period of time. This intermediate step in later cycles of the amplification process reduces the effective concentration of the high copy target nucleic acid available for amplification in later cycles, thereby making more DNA polymerase available for amplification of the low copy target nucleic acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.