Texaphyrin metal complex mediated ester hydrolysis

US5559207A · kind A · utility

Assignees

• The Board of Regents of the University of Texas · US
• Pharmacyclics LLC · US

Inventors

• Jonathan L. Sessler · Austin, US
• Daniel A. Smith · Goshen, US
• Richard A. Miller · Kingsport, US
• Kevin L. Ross · Blue Ash, US
• Meredith Wright · San Jose, US
• William C. Dow · Fremont, US
• Vladimir A. Kr al · Austin, US
• Brent L. Iverson · Austin, US
• Darren Magda · Cupertino, US

Key dates

• Filing date: Apr 14, 1994
• Grant date: Sep 24, 1996
• Expiry date: Apr 14, 2014

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01J2531/96
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A method of phosphate ester hydrolysis including incubating a solution of an aqueous phosphate ester with a texaphyrin metal complex. The metal is a metal cation having catalytic activity for ester bond hydrolysis in aqueous solution, in particular, a lanthanide metal cation, preferably Eu(III) or Dy(III). A preferred substrate is RNA and a preferred texaphyrin is a derivatized texaphyrin having binding specificity, in particular, a texaphyrin covalently coupled to a site-directed molecule, preferably an oligonucleotide.