Promoters from chlorella virus genes providing for expression of genes in prokaryotic and eukaryotic hosts
US5563328A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 27, 1994 |
| Grant date | Oct 8, 1996 |
| Priority date | — |
| Expiry date | Jun 27, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8216
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention is directed to novel promoters or mutants thereof from Chlorella virus DNA methyltransferase genes. A Chlorella virus gene promoter is operably linked to a first and/or second DNA sequence encoding a gene that is different from the Chlorella virus gene to form an expression cassette. An expression cassette can be introduced into prokaryotic and/or eukaryotic cells and can provide for a high level of expression of the gene encoded by the first and/or second DNA sequence. The invention also provides a method for screening other Chlorella virus genes for promoters that can function to express a heterologous gene in prokaryotic and/or eukaryotic hosts.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.