Compounds and methods for site directed mutations in eukaryotic cells
US5565350A · kind A · utility
439Cited by
2References
28Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Dec 9, 1994 |
| Grant date | Oct 15, 1996 |
| Priority date | — |
| Expiry date | Dec 9, 2014 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01K2217/05
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention concerns a polynucleotide having both ribonucleotides and deoxyribonucleotides in a first strand and solely deoxyribonucleotides in a second strand; wherein the strands are Watson-Crick paired and are linked by an oligonucleotide so that the polynucleotide has at most a single 3' and a single 5' end. These ends can be ligated so that the polynucleotide is a single continuous circular polymer. The polynucleotide can be used to induce specific alterations in targeted genes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.