Method and kit for enzymatically determining the pH of a specimen
US5567581A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 6, 1995 |
| Grant date | Oct 22, 1996 |
| Priority date | — |
| Expiry date | Mar 6, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/58
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention concerns a method of enzymatically determining the pH of a specimen (e.g., a solution or a biological fluid) and a kit for conducting the method. The present method involves mixing (1) a specimen with (2) an enzyme and (3) one or more substrates for the enzyme in a buffered solution having a pH effective to provide a direct proportional relationship between the activity of the enzyme and the pH of the specimen; determining the activity of the enzyme; and correlating the activity of the enzyme to the pH of the specimen. Each of the sample, the enzyme, the substrate and the buffered solution is present in an amount effective to provide the direct proportional relationship between the activity of the enzyme and the pH of the specimen. The present kit contains an enzyme, one or more substrates for the enzyme present in an amount effective to determine the enzyme activity, and a buffered solution having a pH effective to provide a direct proportional relationship between the activity of the enzyme and the pH of the specimen. The present invention is particularly applicable to detecting adulteration of a urine specimen.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.