Purification, composition and specificity of heparinase I, II, and III from flavobacterium heparinum
US5569600A · kind A · utility
23Cited by
6References
2Claims
0Family size
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Key dates
| Filing date | Jan 26, 1995 |
| Grant date | Oct 29, 1996 |
| Priority date | — |
| Expiry date | Jan 26, 2015 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/85
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A single, reproducible scheme to simultaneously purify all three of the heparin lyases from F. heparinum to apparent homogeneity is disclosed herein. The kinetic properties of the heparin lyases have been determined as well as the conditions to optimize their activity and stability. Monoclonal antibodies to the three heparinases are also described and are useful for detection, isolation and characterization of the heparinases.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.