Sequencing near infrared and infrared fluorescense labeled DNA for detecting using laser diodes and suitable labels thereof
US5571388A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 1, 1994 |
| Grant date | Nov 5, 1996 |
| Priority date | — |
| Expiry date | Mar 1, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/80
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
To sequence DNA automatically, DNA marked with far infrared, near infrared, or infrared fluorescent dyes are electrophoresed in a plurality of channels through a gel electrophoresis slab or capillary tubes wherein the DNA samples are resolved in accordance with the size of DNA fragments in the gel electrophoresis slab or capillary tubes into fluorescently marked DNA bands. The separated samples are scanned photoelectrically with a laser diode and a sensor, wherein the laser scans with scanning light at a wavelength within the absorbance spectrum of said fluorescently marked DNA samples and light is sensed at the emission wavelength of the marked DNA.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.