Non-infectious, replication defective, immunogenic HIV retrovirus-like particles produced from a recombinant HIV genome devoid of long terminal repeats
US5571712A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 6, 1994 |
| Grant date | Nov 5, 1996 |
| Priority date | — |
| Expiry date | May 6, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S977/924
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
This invention is directed towards nucleic acid molecules capable of producing human immunodeficiency virus (HIV) retrovirus-like particles, which are non-infectious, replication defective, and immunogenic. Recombinant HIV genomes were generated that are devoid of long terminal repeats (LTRs) but contain a heterologous, inducible metallothionein promoter. Additional modifications have been made to the primer binding site, pol, vif, and env coding regions. Upon transfection into a suitable host these DNA molecules are capable of producing HIV retrovirus-like particles that lack genomic RNA. These non-infectious particles will provide suitable antigens for HIV diagnostic assays and immunogenic preparations.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.