DNA/RNA target and signal amplification
US5573914A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | May 15, 1995 |
| Grant date | Nov 12, 1996 |
| Priority date | — |
| Expiry date | May 15, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6865
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A process for detecting the presence or absence of a specific nucleic acid sequence or antibody in a sample using an oligonucleotide to bind to the nucleic acid sequence or antibody to be detected, forming double-stranded nucleic acid sequence using the bound oligonucleotide in conjunction with another oligonucleotide or DNA synthesis, synthesizing RNA transcripts from the thus-formed double-stranded nucleic acid sequence, and detecting the existence of the RNA transcripts, and oligonucleotides and kits useful in carrying out such a process.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.