Immunological detection method for triazines

US5573922A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Eva Hoess · Starnberg, DE
• Erasmus Huber · Garching bei München, DE
• Christine Markert-Hahn · Penzberg, DE
• Wolfgang Rollinger · Polling, DE

Key dates

• Filing date: Apr 29, 1994
• Grant date: Nov 12, 1996
• Expiry date: Apr 29, 2014

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/815
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

An immunological method for the determination of triazine and triazine derivatives by a competitive immunoassay in which the sample and labelled atrazine compete for a polyclonal antibody which is bound to a solid phase before, during or after the immunological reaction, the immunological complex binds to the solid phase, the solid phase and unbound labelled atrazine are separated and the label in the solid or liquid phase is determined as a measure of the content of triazine and triazine derivatives, which is characterized in that polyclonal antibodies which were obtained by immunization with a 4-alkylamino-S-triazine are used and a conjugate of a label and 4-amino-S-triazine which is bound to the label via the 6 position is used as the labelled atrazine. Triazine and triazine derivatives can be determined simultaneously using this method.