Functionalized carrier materials for the simultaneous synthesis and direct labeling of oligonucleotides as primers for template-dependent enzymatic nucleic acid syntheses

US5574141A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Hartmut Seliger · Neu-Ulm, DE
• Wojciech T. Markiewicz · Poznań, PL
• Gabriele Gr oger · Langenau, DE
• Rudi R osch · Ulm, DE
• Margit Klotz · Schelklingen, DE

Key dates

• Filing date: Feb 28, 1994
• Grant date: Nov 12, 1996
• Expiry date: Feb 28, 2014

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07H21/00
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

The synthesis and use of polymeric carriers is described which are loaded with nucleic acid building blocks which in turn contain labelling groups or precursors thereof. The polymeric carrier loaded in this way serves as a solid or liquid phase for the assembly of oligonucleotides which can be used as primers for template-dependent enzymatic nucleic acid syntheses such as for example in sequencing analysis according to Sanger and co-workers or in the polymerase chain reaction (PCR).