Human Kunitz-type protease inhibitor variant

US5576294A · kind A · utility

Assignee

• Novo Nordisk A/S · DK

Inventors

• Fanny Norris · Hellerup, DK
• Kjeld Norris · Copenhagen, DK
• S.o slashed.ren E. Bj.o slashed.rn · Avenue Madeleine, DK
• Lars Christian Petersen · Hørsholm, DK
• Ole Hvilsted Olsen · Hjørring, DK

Key dates

• Filing date: Oct 12, 1994
• Grant date: Nov 19, 1996
• Expiry date: Oct 12, 2014

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K38/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Variant of human Kunitz-type protease inhibitor domain II of tissue factor pathway inhibitor (TFPI), the variant comprising the following amino acid sequence ##STR1## wherein Xaa at position 1 is H or a naturally occurring amino acid residue except Cys, each Xaa at positions 2-5 is independently a naturally occurring amino acid residue except Cys or is absent, each Xaa at positions 14, 16, 18, 19, 20, 21, 22, 23, 37, 42, 43, 44, and 49 independently a naturally occurring amino acid except Cys, each Xaa at positions 61, 62, 63, and 64 is independently a naturally occurring amino acid except Cys or is absent, and Xaa at position 65 is OH or a naturally occurring amino acid except Cys, with the proviso that at least one of the amino acid residues designated Xaa is different from the amino acid residue of the native sequence.