Chemiluminescent dialkyl-substituted 1,2-dioxetane compounds, methods of synthesis and use
US5578253A · kind A · utility
12Cited by
11References
35Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 23, 1994 |
| Grant date | Nov 26, 1996 |
| Priority date | — |
| Expiry date | Nov 23, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07C2601/14
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A chemiluminescent assay method and compositions are described which use a dialkyl-substituted dioxetane which is deprotected to trigger a chemiluminescent reaction. Chemiluminescent 1,2-dioxetane compounds substituted on the dioxetane ring with two nonspirofused alkyl groups which can be triggered by a reagent to generate light are disclosed. Dialkyl-substituted dioxetanes are useful for the detection of triggering agents including enzymes. The enzyme may be present alone or linked to a member of a specific binding pair in an immunoassay, DNA probe assay or other assay where the enzyme is bound to a reporter molecule.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.