In vitro method of evaluating the effects of a substance
US5578445A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 6, 1994 |
| Grant date | Nov 26, 1996 |
| Priority date | — |
| Expiry date | Apr 6, 2014 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/74
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An in vitro method of evaluating the antagonistic versus agonistic effects of a receptor-binding test substance on a selected type of cells containing endogenous intra-cellular hormone receptors, is disclosed. Said test substance, and separately a reference substance, known to be either an antagonist or an agonist, are incubated with said selected type of cells, and the magnitude of the selected cellular response resulting from hormone/receptor interaction, is analyzed. In case the method is performed on at least two selected types of cells which derive from different kinds of tissues, it is possible to evaluate the pattern of antagonistic versus agonistic effects of the selected test substance on said kinds of tissues.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.