Enhancement of immunochemical staining in aldehyde-fixed tissues
US5578452A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 7, 1994 |
| Grant date | Nov 26, 1996 |
| Priority date | — |
| Expiry date | Apr 7, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/25125
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for restoring immunoreactivity of a tissue, particularly decalcified tissue, fixed width an aldehyde fixative agent and embedded in an embedding medium, usually comprising celloidin, the method comprising the steps of contacting the tissue with an aldehyde releasing reagent solution comprising a solvent and an aldehyde releasing reagent and removing aldehyde released by the aldehyde releasing reagent from contact with the tissue by reacting the aldehyde in a substantially irreversible manner to form a non-aldehyde derivative, and removing excess base from the tissue. A preferred solution for celloidin-embedded decalcified tissue comprises methanolic sodium hydroxide at about one-third saturation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.