Recombinant co-expression system of protein disulfide isomerase gene, yeast receptor protein ERD2 gene and a foreign product polypeptide gene, and a process for producing the foreign polypeptide using such system
US5578466A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 17, 1992 |
| Grant date | Nov 26, 1996 |
| Priority date | — |
| Expiry date | Apr 17, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is directed to a transformant comprising the following expression units in a co-expressible state: PA1 an expression unit containing a gene coding for a receptor protein ERD2 from yeast or analog thereof which is capable of binding to a protein localizing in endoplasmic reticulum and having a signal for staying therein; PA1 an expression unit containing a gene coding for said protein localizing in endoplasmic reticulum; and PA1 an expression unit containing a foreign gene coding for a polypeptide which is a subject of function of said protein localizing in endoplasmic reticulum, and to a transformant comprising, in a co-expressible state, a fusion gene which is composed of a DNA fragment coding for a human serum albumin prepro-sequence and a foreign gene coding for a useful polypeptide. The present invention is also directed to a process for producing said polypeptide by co-expressing said genes in said transformant such that the polypeptide is predominantly secreted out of the transformant cell. Consequently, the invention has an advantage of improving the productivity of said polypeptide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.