Use of deoxyinosine containing primers to balance primer efficiency in the amplification of nucleic acid molecules
US5578467A · kind A · utility
34Cited by
2References
16Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 20, 1994 |
| Grant date | Nov 26, 1996 |
| Priority date | — |
| Expiry date | May 20, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/156
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides improved methods for amplifying a nucleic acid molecule. Specifically, the invention involves replacing at least one nucleotide of an oligonucleotide with a deoxyinosine residue, so as to more equalize the efficiency with which that oligonucloetide and a second oligonucleotide hybridize to a target molecule, and then amplifying the target molecule using, for example, the polymerase chain reaction. Improved amplification results from the improvement in the relative hybridization efficiencies.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.