Nickel-free hemoglobin and methods for producing such hemoglobin
US5578564A · kind A · utility
12Cited by
19References
30Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jul 23, 1993 |
| Grant date | Nov 26, 1996 |
| Priority date | — |
| Expiry date | Jul 23, 2013 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K38/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
According to the present invention removal of nickel from a nickel-containing hemoglobin solution is accomplished by exposing the nickel-containing hemoglobin solution to a chelating agent, preferably a multidentate acetic acid based chelator such as EDTA. Methods are provided that allow obtaining essentially nickel-free hemoglobin from nickel-containing hemoglobin solutions, particularly hemoglobin solutions that result from large scale fermentation and/or purification.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.