Method for the detection of proteins containing phosphorylated tyrosine

US5580742A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Heinz Bodenm uller · Tutzing, DE
• Andreas Dessauer · Tutzing, DE

Key dates

• Filing date: Jan 21, 1994
• Grant date: Dec 3, 1996
• Expiry date: Jan 21, 2014

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/822
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

For the detection of proteins containing phosphorylated tyrosine the sample of a body fluid is incubated with at least two receptors R.sub.1 and R.sub.2 whereby a change in signal is produced by binding of at least the receptors R.sub.1 and R.sub.2 with the substance to be detected in the sample solution and in which in each case one of the two receptors contains an antibody or its derivative capable of specifically binding to the protein to be detected and the other receptor contains an antibody or its derivative capable of specifically binding to phosphorylated tyrosine and the signal change caused by the binding is determined in the sample.