Method for the determination of HDL cholesterol by means of a rapid diagnostic agent with an integrated fractionating step

US5580743A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Walter Rittersdorf · Mannheim, DE
• Ulfert Deneke · Fürth, DE
• Gerhard Hiller · Mannheim, DE
• Hartmut Merdes · Heidelberg, DE
• Klaus Buecker · Viernheim, DE
• Uwe Goebbert · Mannheim, DE

Key dates

• Filing date: Feb 6, 1995
• Grant date: Dec 3, 1996
• Expiry date: Feb 6, 2015

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/255
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention relates to a method useful in separating non-high density lipoproteins, referred to as "non-HDLs", from biological fluids containing them. A porous carrier is provided which contains a non-HDL precipitating agent. One need only contact the sample of interest to the carrier for one minute or less, after which the precipitated non-HDLs are no longer present in the sample being tested. The applications of the method include the ability to determine high density lipoproteins in the sample without interference from non-HDLs.