Cloning vector for use in lactic acid bacteria
US5580787A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Aug 13, 1992 |
| Grant date | Dec 3, 1996 |
| Priority date | — |
| Expiry date | Aug 13, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/746
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A recombinant plasmid useful as a cloning vector in lactic acid bacteria and only containing DNA of lactic acid bacterial origin, the plasmid comprising (a) a DNA fragment comprising a replication region functional in lactic acid bacteria and (b) a DNA fragment comprising a marker gene selectable in lactic bacteria, the expression of which allows one-step primary selection in lactic acid bacterial cells transformed with the recombinant plasmid; a method for constructing the cloning vector plasmid; the recombinant plasmid with an inserted gene coding for a desired gene product; a method of preparing an improved food starter culture by introducing the recombinant plasmid with an inserted gene coding for a desired gene product and food starter cultures prepared by the method are provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.