Multiplexed capillary electrophoresis system
US5582705A · kind A · utility
131Cited by
29References
49Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 19, 1995 |
| Grant date | Dec 10, 1996 |
| Priority date | — |
| Expiry date | May 19, 2015 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44726
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention provides a side-entry optical excitation geometry for use in a multiplexed capillary electrophoresis system. A charge-injection device is optically coupled to capillaries in the array such that the interior of a capillary is imaged onto only one pixel. In Sanger-type 4-label DNA sequencing reactions, nucleotide identification ("base calling") is improved by using two long-pass filters to split fluorescence emission into two emission channels. A binary poly(ethyleneoxide) matrix is used in the electrophoretic separations.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.