Chemiluminescent 1,2-dioxetanes
US5582980A · kind A · utility
18Cited by
6References
6Claims
0Family size
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Key dates
| Filing date | Apr 25, 1994 |
| Grant date | Dec 10, 1996 |
| Priority date | — |
| Expiry date | Apr 25, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/975
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Spiroadamantyl dioxetanes bearing an alkoxy substituent, and a phenyl substituted on the dioxetane ring can be activated to chemiluminesce if the aromatic substituent bears a meta-substituted moiety designated OX, wherein the X is cleaved by an enzyme with which the dioxetane is permitted to come in contact with. The T.sub.1/2 kinetics of the chemiluminescent reaction, as well as the signal intensity and/or quantum yield of the chemiluminescent reaction, can be altered by addition of a chlorine substituent at position 4 on the phenyl ring. Signal strength can further be enhanced by recognized chemiluminescent enhancers.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.