Methods of use of phenanthridium DNA intercalators for fluorescence detection
US5582984A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 29, 1994 |
| Grant date | Dec 10, 1996 |
| Priority date | — |
| Expiry date | Jun 29, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07D417/06
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Phenanthridium compounds are provided which are comprised of intercalators and substituted intercalator segments having a functionalized chain or chains which provide a high affinity for binding to the DNA molecule and show reduced self-quenching, while providing superior transport kinetics. The compounds have been found to provide enhanced fluorescence when bound to a DNA molecule within a fluorescent flow cytometry environment which is about eight to ten times brighter in fluorescence than "bis" structure intercalators and other known intercalators utilized in flow cytometry environment, said enhancement of fluorescence allows a drastic reduction in the amount of intercalator needed for applications of similar sensitivity than those using known intercalators. Substantial improvements in material use efficiency, accuracy and dependability of various DNA studies are direct results of the inventive intercalator compounds, said compounds having use in known DNA intercalator applications inclusive of flow cytometry, gel electrophoresis, DNA detection, immunoassay for DNA, fluorescence in situ hybridization assays, DNA sequencing application, therapeutic application and conversion stai…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.