Determination and detection of antibody and its immunoglobulin class
US5583054A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 26, 1994 |
| Grant date | Dec 10, 1996 |
| Priority date | — |
| Expiry date | Sep 26, 2014 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/92
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides a method for determining the presence of a class of an antibody in a biological sample. In this method, a first reagent including insoluble particles having an antigen to the antibody immobilized on the surface thereof, and a second reagent including insoluble magnetic particles having immobilized on the surface thereof a substance particularly reactive to a specific immunoglobulin class, is reacted with the sample under conditions to promote agglutination of the first and second reagents with the antibody. The unreacted second reagent and the agglutinate are separated from the unreacted first reagent by application of a magnetic field. Then the amount of unreacted first reagent is determined.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.