Extracellular serine protease and a Bacillus subtilis alkaline neutral an serine protease mutant strain

US5585253A · kind A · utility

Assignee

• The Regents of the University of California · US

Inventors

• Roy H. Doi · Davis, US
• Lin Wang · University City, US
• Reinhold Br uckner · Herrenberg, DE

Key dates

• Filing date: Nov 27, 1989
• Grant date: Dec 17, 1996
• Expiry date: Nov 27, 2009

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/75
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A cloned gene (epr) encoding a novel extracellular protease, Epr, from Bacillus subtilis is described. Also described is a triple extracellular neutral, alkaline and serine protease deficient Bacillus subtilis mutant strain having deletions in the (npr), (apr) and (epr) genes encoding these proteases. The triple mutant strain was constructed by the gene conversion technique and produces about 1% of the extracellular proteolytic activity of the wild type. It is a particularly useful host for the production of heterologous proteins that are secreted into the growth medium.