Process for the production of a viable cell culture infected by a multiple sclerosis-associated virus
US5585262A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Feb 2, 1994 |
| Grant date | Dec 17, 1996 |
| Priority date | — |
| Expiry date | Feb 2, 2014 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2469/20
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to a process for in vitro culture of cells infected by a virus associated with multiple sclerosis and to the infected cell lines thus produced. The process is the cultivation of human cells infected by a viral strain to obtain at least one culture of primary cells infected by the said viral strain, along with the cultivation of non-infected human cells permissive to the viral strain to obtain at least one permissive culture, followed by cocultivation of at least one sample of a culture of infected primary cells and one sample of the permissive culture to obtain a first infected derived culture, then cultivating in series of the first infected derived culture. The invention is used in particular in the pharmaceutical diagnostics industry sector. In the preferred process, the infected cells are leptomeningeal cells and the permissive cells are leptomeningeal cells or plexuschoroideus cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.