Purified retroviral constitutive transport enhancer and its use to facilitate mRNA transport, and to produce recombinant, attenuated HIV

US5585263A · kind A · utility

Assignees

• The University of Alabama at Birmingham Research Foundation · US
• Research Foundation for the State University of New York York · US

Inventors

• Marie-Louise Hammarskj old · Earlysville, US
• David Rekosh · Earlysville, US
• Molly Bray · Charlottesville, US
• Eric Hunter · Birmingham, US

Key dates

• Filing date: May 20, 1994
• Grant date: Dec 17, 1996
• Expiry date: May 20, 2014

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2830/48
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A novel retroviral nucleotide sequence comprising a constitutive transport enhancer which functions to transport mRNA transcripts from the nucleus to the cytoplasm of a cell, wherein the mRNA transcript is either differentially spliced, alternatively spliced, incompletely spliced, or unspliced. Also disclosed is a recombinant attenuated HIV containing only structural proteins gag, pol, and env, wherein the expression of the structural proteins is rev-independent and facilitated by the constitutive transport enhancer.