Methods and compositions for detecting base pair mismatches
US5589329A · kind A · utility
13Cited by
2References
44Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 15, 1993 |
| Grant date | Dec 31, 1996 |
| Priority date | — |
| Expiry date | Nov 15, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention discloses improved compositions and methods for detecting mutations, including single base changes, in nucleic acid sequences using RNase protection assays. The improvements include concomitant, dramatic reductions in the salt and RNase enzyme concentrations in the RNase digestion reaction mixture which result in greater sensitivity in detecting genetic mutations. Another embodiment of the present invention is kits to be used for the detection of single base mismatches in nucleic acid samples.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.