Hybridization promotion reagents
US5589335A · kind A · utility
24Cited by
0References
12Claims
0Family size
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Key dates
| Filing date | Aug 11, 1994 |
| Grant date | Dec 31, 1996 |
| Priority date | — |
| Expiry date | Aug 11, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/967
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Novel reagents are provided along with methods for their use in nucleic acid hybridization assays for detecting target nucleic acids in samples. The most preferred reagent is a mixture of guanidinium thiocyanate and tetramethylammonium:Trifluoracetate and possesses many properties for facilitating hybridization between target nucleic acid and nucleic acid probes capable of binding thereto and surprisingly results in the phenomenon of superstoichiometric labeling.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.