Method for mammalian cell transfection
US5589368A · kind A · utility
1Cited by
1References
23Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 25, 1994 |
| Grant date | Dec 31, 1996 |
| Priority date | — |
| Expiry date | Apr 25, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/948
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a process for transfecting a mammalian cell culture. The process includes incubating a cell culture in the presence of a transfection medium that includes a serum that is of a different type from the serum used in the normal growth medium used to grow the cell culture. It is preferred that the normal growth medium include fetal bovine serum and the transfection medium include a serum such as human, calf, horse, lamb, or pig. The transfection medium may further include an hydryoxylated sterol such as 25-hydroxycholesterol.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.