Natamycin recovery
US5591438A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 3, 1994 |
| Grant date | Jan 7, 1997 |
| Priority date | — |
| Expiry date | May 3, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P19/626
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for the recovery of high purity natamycin from fermentaion broth by extraction with methanol is disclosed. In one embodiment the process comprises the steps: PA0 (1) adding methanol to a natamycin feed stream comprising at least 2 g/L of solid suspended natamycin to form an extraction medium, and maintaining the extraction medium at a temperature of 0.degree.-25.degree. C., preferably not over 15.degree. C.; PA0 (2) adjusting the pH of the extraction medium to 1.0 to 4.5 while maintaining the temperature at 0.degree.-25.degree. C. for 0.5 to 30 hours, but preferably not over 0.5 hours when the temperature is 15.degree.-25.degree. C.; PA0 (3) removing solids from the extraction medium to form an extraction liquor; PA0 (4) raising the pH of the extraction liquor to 6.0-9.0; and PA0 (5) recovering precipitated natamycin.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.