Electrochemiluminescent rhenium moieties and methods for their use

US5591581A · kind A · utility

Assignee

• IGEN Co., LTD · US

Inventors

• Richard J. Massey · Rockville, US
• Michael J. Powell · Gaithersburg, US
• Walter J. Dressick · Fort Washington, US
• Jonathan K. Leland · Silver Spring, US
• Janel K. Hino · Arlington, US
• Mohindar S. Poonian · West Caldwell, US
• Leopoldo Della Ciana · Gaithersburg, US

Key dates

• Filing date: Apr 15, 1994
• Grant date: Jan 7, 1997
• Expiry date: Apr 15, 2014

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/81
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Electrochemiluminescent moieties having the formula EQU (Re(P).sub.m (L.sup.1).sub.n (L.sup.2).sub.o (L.sup.3).sub.p (L.sup.4).sub.t (B).sub.u wherein PA1 P is a polydentate ligand of Re; PA1 L.sup.1, L.sup.2, L.sup.3 and L.sup.4 are ligands of Re, each of which may be the same as or not the same as each other ligand; PA1 B is a substance which is a ligand of Re or is conjugated to one or more of P, L.sup.1, L.sup.2, L.sup.3 and L.sup.4 ; PA1 m is an integer equal to or greater than 1; PA1 each of n, o, p, q, r and s is zero or an integer; PA1 t is an integer equal to or greater than 1; and PA1 u is an integer equal to or greater than 1; PA1 P, L.sup.1, L.sup.2, L.sup.3, L.sup.4 and B being of such composition and number that the chemical moiety can be induced to electrochemiluminesce and the total number of bonds to Re provided by the ligands of Re being equal to the coordination number of Re are disclosed. Qualitative and quantitative electrochemiluminescent assays for analytes of interest present in multicomponent liquids using these moieties are also disclosed.