Method for quantitating hydrazide groups
US5591598A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 22, 1995 |
| Grant date | Jan 7, 1997 |
| Priority date | — |
| Expiry date | Feb 22, 2015 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/101666
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method is provided for the preparation of semisolid particles or cells that have been chemically derivatized resulting in hydrazide functionalities covalently incorporated onto cell surface membrane molecules of the semisolid particles or cells, as well as a method for determining the levels of hydrazide groups on the particles or cells. The method involves the preparation and analysis of red blood cells chemically derivatized in such a manner that hydrazide functionalities have been covalently incorporated onto the cell membrane molecules, the preparation of hydrazine derivatized cells and the use of the novel cells in agglutination reactions to promote the attachment of antigens and antibodies to the hydrazine derivatized cells without loss of antibody activity or the blocking of the epitope of interest.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.