Functional DNA block and plasmid coding for hirudin, transformed yeast, method for preparing hirudin, hirudin obtained and its pharmaceutical use
US5591640A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 16, 1995 |
| Grant date | Jan 7, 1997 |
| Priority date | — |
| Expiry date | Mar 16, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a functional DNA block by means of which hirudin may be prepared from yeast, which block contains at least the sequence: EQU --S.sub.tr --L.sub.ex --S.sub.cl --H gene PA1 H gene being the gene coding for hirudin or one of its variants, PA1 S.sub.tr is a DNA sequence containing the signals which provide for the transcription of the H gene by yeast, PA1 L.sub.ex is a leader sequence needed in order to obtain the excretion of the gene product, PA1 S.sub.cl is a DNA sequence coding for the site of cleavage by yscF proteinase, which cleavage site is unique and situated in the hirudin precursor immediately upstream from the H gene, The invention relates more especially to a functional DNA block in which the H gene codes for HV1.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.