Rapid purification of circular DNA by triplex-mediated affinity capture
US5591841A · kind A · utility
20Cited by
2References
10Claims
0Family size
Inventors
Key dates
| Filing date | Sep 30, 1994 |
| Grant date | Jan 7, 1997 |
| Priority date | — |
| Expiry date | Sep 30, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1003
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A single-step capture of a target supercoiled double-stranded DNA molecule is accomplished by forming a local triple-helix among two strands of the supercoiled circular DNA and an oligonucleotide probe. The oligonucleotide is bound to an immobilizing support which facilitates the immobilization and purification of target DNA molecules. Non-target DNA molecules and other contaminating cellular material are easily removed by washing. The triple-helical structure is destabilized by raising the pH, leaving purified target DNA in the supernatant and reusable affinity capture oligonucleotide secured to the immobilizing support.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.