DNA sequence-based HLA class I typing method
US5593830A · kind A · utility
4Cited by
1References
19Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jul 28, 1994 |
| Grant date | Jan 14, 1997 |
| Priority date | — |
| Expiry date | Jul 28, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/172
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a process for determining genotypes in highly polymorphic systems by polymerase chain reaction amplification of cDNA or genomic DNA and direct sequencing polymerase chain reaction products using oligonucleotide primers. More specifically, Class I HLA genotypes can be unambiguously determined in any subject in 16-24 hours by direct sequencing of HLA-A, HLA-B, and HLA-C transcripts enzymatically amplified and sequenced using a limited number of selected oligonucleotide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.