Enzymatic oxidation of alcohols to aldehydes in a continuous reaction system using Candida boidinii
US5593872A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 8, 1995 |
| Grant date | Jan 14, 1997 |
| Priority date | — |
| Expiry date | Sep 8, 2015 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/921
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for the enzymatic oxidation of alcohols to aldehydes in a continuous reaction series is disclosed utilizing crude cellular methanol oxidase and catalase produced by an acceptable organism. The process maintains a steady-state aldehyde concentration whereby enzyme activity is promoted and side reactions are eliminated. In particular, the process has two reactors operating in series continuously producing a fermenter effluent containing growing, intact Candida boidinii cells at a cell concentration of about 0.5 to about 3% by weight of the effluent by continuous culture of the cells on a methanol carbon source, continuously introducing the effluent from the first reactor to the second reactor, adding an alcohol feed at about 0.2-10% w/w and oxygen to the second reactor, maintaining a steady-state aldehyde and alcohol concentration in the reaction mixture, the concentration of residual alcohol being about 0.1-9% w/w by controlling the rates of introduction of effluent and alcohol feed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.